Multidisciplinary Cancer Investigation

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Derogar P, Abolfathi A A, Safi Samghabadi A, Yazdani A, Afkhami F. A Comparison Between Malignant Breast Neoplasms Apoptosis and Benign Breast Neoplasms Under Influence of DNA Fragmentation. Multidiscip Cancer Investig 2017; 1
URL: http://mcijournal.com/article-1-85-en.html

Introduction: Interrelationship of cells proliferation and apoptosis regulate homeostasis in normal tissues. Breast cancer development is mainly due to defective apoptosis pathway. Since there is a significant interrelationship between the ratio of apoptotic bodies to normal cells and breast cancer pathogenesis, we conducted this study to contrast malignant breast neoplasms apoptosis with benign breast neoplasms under the influence of DNA Fragmentation.
Materials and Methods: Sixty patients entered this study. Its control group were their both benign neoplasms and normal cells. After DNA extraction, DNA fragmentation was carried out with DNA laddering assay. Apoptotic bodies were detected in 1% agarose gel electrophoresis.
Results: Apoptotic bodies were detected in 83.3% of breast cancer samples. However, no sign of apoptosis was observed in control samples and the number of positive cases in malignant samples was significantly higher than benign samples (P< 0.05).
Conclusions: It is revealed that the rate of apoptosis detectable by DNA fragmentation in malignant breast neoplasms is higher than benign types so that it can be used as a quick-diagnostic technique.