en Materials Science and Nanomedicine Original/Research Article Introduction: Frizzled family receptor 7 (FZD7) is one of the 10 members of Frizzled<br> receptors. It interacts with Wnt ligands to activate canonical wnt signaling, which turns on<br> different downstream transcription factors essential for modulating cellular proliferation,<br> polarity, and differentiation. Altered expression of FZD7 receptor is associated with development<br> and progression of many cancers including breast cancer. An effective targeted<br> therapy for breast cancer through modulating ligand-receptor interaction may involve the<br> use of antibodies to antagonize FZD7. ScFvs (single-chain fragment variable) have provided<br> an alternative to full-length monoclonal antibodies (mAbs) in diagnostic and therapeutic<br> applications.<br> Materials and Methods: A phage antibody library of scFv was used and selection of specific<br> scFvs were performed by 4 rounds of panning process against an immunodominant epitope<br> of FZD7, followed by PCR and fingerprinting of the selected clones. ELISA was used<br> to confirm the specificity of the clones. Apoptotic effects of the selected scFv on MDAMB-<br> 231 cell line were assessed by annexin V/PI assay after 24 h and 48 h.<br> Results: A specific scFv with the frequency of 35% was isolated which produced positive<br> ELISA with the corresponding epitope. After 24h treatment with the selected scFv,<br> MDA-MB-231 cells showed 48.7% apoptotic cell death (Annexin V+/PI&minus;). However, this<br> amount increased to 81.6% following 48h treatment with scFv.<br> Conclusions: Due to unique apoptotic properties of selected scFv including human origin,<br> high affinity and specificity, this agent has been applied in cancer immunotherapy. The<br> specific anti-FZD7 scFv selected in this study has the potential to be used for inhibiting<br> wnt signaling pathway in breast cancer cells. Anti-FZD scFv Frizzled Receptor 7 Breast Cancer 0 0 http://mcijournal.com/browse.php?a_code=A-10-33-39&slc_lang=en&sid=1 Neda Zarei 1003194753284600724 1003194753284600724 Yes Foroogh Nejatollahi 1003194753284600725 1003194753284600725 No