Multidisciplinary Cancer Investigation
نشریه بین المللی چند تخصصی سرطان
Multidiscip Cancer Investig
Medical Sciences
http://mcijournal.com
1
admin
2476-4922
2538-1911
10.30699/acadpub.mci
en
jalali
1396
7
1
gregorian
2017
10
1
1
Supplement Issue 1: Abstracts of International Tehran Breast Cancer Congress
online
1
fulltext
en
Prediction of Hub Transcription Factors of Breast Cancer Cells Response to Cisplatin Treatment
Materials Science and Nanomedicine
Original/Research Article
<div style="text-align: justify;"><strong>Introduction:</strong> Hub genes are connected to many other genes by encoding proteins that interact directly with DNA molecules or other proteins. These hubs can act as transcription factors more than controlling expression of genes. Finding these genes is substantial in detection of the main controller of the pathways of diseases such as cancer, designing treatments regarding them and furthermore a probable direct treatment to the disease.<br>
<strong>Materials and Methods:</strong> In this study, we used a dataset (GSE28274) of treated breast cancer cell line MCF7 via cisplatin. We analyzed data of cells in glycolysis and impedance stages versus control cells using GEO2R to determine significantly (p-value less than 0.05) differentially expressed genes. Threshold for choosing the genes were amounts of ±1.2 of log2 Fold change. Then, we detected transcription factors of differentially expressed genes using ChEA database. Networks of differentially expressed transcription factors were constructed and functional annotations and biological pathways were detected by DAVID database.<br>
<strong>Results: </strong>Our results showed that there are 9 and 13 differentially expressed transcription factors in glycolysis and impedance stages of the treated cells, respectively. In both stages there were six identical transcription factors with similar expression patterns except for PPARA. Annotation assignments revealed that the transcription factors are related to gene expression process, signaling and cancer pathways.<br>
<strong>Conclusions:</strong> In conclusion, our study is offering some potential transcription factors as hub genes that affect gene expression processes and cancer pathways.</div>
Breast Cancer, Cisplatin, Transcription Factors, Prediction, Hub Genes
0
0
http://mcijournal.com/browse.php?a_code=A-10-33-47&slc_lang=en&sid=1
Rasoul
Godini
1003194753284600749
1003194753284600749
Yes
Hossein
Fallahi
1003194753284600750
1003194753284600750
No