Multidisciplinary Cancer Investigation
نشریه بین المللی چند تخصصی سرطان
Multidiscip Cancer Investig
Medical Sciences
http://mcijournal.com
1
admin
2476-4922
2538-1911
10.30699/acadpub.mci
en
jalali
1397
4
1
gregorian
2018
7
1
2
3
online
1
fulltext
en
Study of Association Between TLR4 D299G and T399I Polymorphisms and Risk of Gastric Cancer
Materials Science and Nanomedicine
Original/Research Article
<div style="text-align: justify;"><strong>Introduction:</strong>Gastrointestinal cancers constitute more than one-third of the most common cancers and half of the fatal cancers worldwide. Toll-like receptors (TLRs) are identified as pivotal receptors in innate immunity responses. TLR4 is the main receptor that plays a role in lipopolysaccharide (LPS) sensing of gram-positive bacteria. D299G (rs4986790) and T399I (rs4986791) polymorphisms in TLR4 lead to a decrease in immune response against LPS. The current study aimed at investigating the relationship between D299G and T399I polymorphisms and susceptibility to gastritis and gastric precancerous lesions in patients referred to Imam Reza Hospital, Tehran, Iran.<br>
<strong>Methods:</strong>The current case-control study was conducted on 201 individuals consisting of 90 patients with gastric cancer (GC) and 111 healthy controls. Genomic DNA was extracted from peripheral blood and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the mentioned polymorphisms.<br>
<strong>Results:</strong>Allelic frequencies and genetic distribution of polymorphisms were analyzed in the patient and control groups. Although 399C and 299A allele frequencies were higher in the patients` group, the difference was not statistically significant (P > 0.05).<br>
<strong>Conclusions:</strong> No significant association was observed between TLR4 polymorphisms on positions 299 and 399, and susceptibility to GC. Also no significant correlation was observed between these two polymorphisms and precancerous lesions. The current study results showed<br>
that the studied polymorphisms cannot be used as a prognostic marker of GC in Iranian population.</div>
Stomach Neoplasms, TLR4, Polymorphism, Single Nucleotide
16
22
http://mcijournal.com/browse.php?a_code=A-10-197-1&slc_lang=en&sid=1
Hasan
Hatami
dr_hatami@yahoo.com
10031947532846001769
10031947532846001769
No
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran
Hamed
Naghoosi
h.naghoosi@gmail.com
10031947532846001770
10031947532846001770
No
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran
Mahyar
Nourian
mahyarnourian1369@gmail.com
10031947532846001771
10031947532846001771
No
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran
Mostafa
Iranpour
m.iranpour@sbmu.ac.ir
10031947532846001772
10031947532846001772
No
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran
Sandra
Saidi
saidisandra699@gmail.com
10031947532846001773
10031947532846001773
No
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran
Mahmoud Reza
Hashemi
hashemim891@yahoo.com
10031947532846001774
10031947532846001774
No
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran
Shahrokh
Iravani
iravani.shahrokh@yahoo.com
10031947532846001775
10031947532846001775
Yes
AJA Cancer Epidemiology Research and Treatment Center (AJA- CERTC), AJA University of Medical Sciences, Tehran, Iran