Multidisciplinary Cancer Investigation
نشریه بین المللی چند تخصصی سرطان
Multidiscip Cancer Investig
Medical Sciences
http://mcijournal.com
1
admin
2476-4922
2538-1911
10.30699/acadpub.mci
en
jalali
1400
12
1
gregorian
2022
3
1
6
1
online
1
fulltext
en
Effects of 1Hz 100mT electromagnetic field on apoptosis induction and Bax/Bcl-2 expression ratio in breast cancer cells
Original/Research Article
<p style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><strong><span style="font-size:10.5pt"><span style="line-height:150%">Introduction</span></span></strong><span style="font-size:10.5pt"><span style="line-height:150%">: Breast cancer is the most common metastatic malignancy and the second leading cause of death in women. Recently, extremely low-frequency electromagnetic fields (ELF-EMFs) seem to modulate the rate of proliferation and enhance apoptosis and are considered as an emerging approach to cancer therapy. Despite recent success in the electromagnetic fields, the results are still neither definitive nor even contradictory. <br>
<b><span style="color:#1d1d1b">Methods: </span></b><span style="color:#1d1d1b">In this </span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span arial="" ms="" style="font-family:" unicode=""><span style="color:#1d1d1b"><span style="letter-spacing:-.2pt">st</span></span></span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span style="color:#1d1d1b"><span style="letter-spacing:-.2pt">udy</span></span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span style="color:#1d1d1b"><span style="letter-spacing:-.15pt">,</span></span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span style="color:#1d1d1b"> induction of apoptosis was considered as one of the possible mechanisms of ELF-EMFs on cancer inhibition. Brea</span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span arial="" ms="" style="font-family:" unicode=""><span style="color:#1d1d1b">st</span></span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span style="color:#1d1d1b"> cancer cell lines were exposed to a 1 Hz, 100mT ELF-EMF (2h/day) for five days. the apoptosis rare of both the exposure and sham exposure groups was determined by flow cytometry. The expression levels of Bax and Bcl-2 were evaluated by real-time PCR.</span></span></span><br>
<span style="font-size:10.5pt"><span style="line-height:150%"><b><span style="color:#1d1d1b">Results: </span></b><span style="color:#1d1d1b">The mRNA expression levels of Bax were increased; while the expression of Bcl-2 showed a decrease in MDA-MB231 cells exposed to 1Hz EMF compared with sham exposure. Moreover, the ratio of Bax/Bcl2 was significantly increased in comparison to the sham exposure. The increased Bax/Bcl-2 ratio induces cell apoptosis.<br>
<b>Conclusions: </b>It is sugge</span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span arial="" ms="" style="font-family:" unicode=""><span style="color:#1d1d1b">st</span></span></span></span><span style="font-size:10.5pt"><span style="line-height:150%"><span style="color:#1d1d1b">ed that ELF-EMF is a new adjuvant therapeutical method that may contribute to anti-cancer and cancer therapy research.</span></span></span></span></span></span></p>
<div style="text-align: justify;"><br>
<span style="font-size:11pt"><span style="punctuation-wrap:simple"><span new="" roman="" style="font-family:" times=""><span style="font-size:3.5pt"></span></span></span></span></div>
Breast Cancer, Apoptosis, Electromagnetic Fields, Bax/Bcl-2
1
7
http://mcijournal.com/browse.php?a_code=A-10-32-2&slc_lang=en&sid=1
Maryam
Moori
10031947532846003270
10031947532846003270
No
Department of Biology, Science and Research Branch, Islamic Azad Univer- sity, Tehran, Iran
Dariush
Norouzian
10031947532846003271
10031947532846003271
No
Pilot Nanobiotechnology Department, Pasteur Institute of Iran, Tehran, Iran
Parichehr
Yaghmaei
10031947532846003272
10031947532846003272
No
Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Leila
Farahmand
laylafarahmand@gmail.com
10031947532846003273
10031947532846003273
Yes
3Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran