Multidisciplinary Cancer Investigation
نشریه بین المللی چند تخصصی سرطان
Multidiscip Cancer Investig
Medical Sciences
http://mcijournal.com
1
admin
2476-4922
2538-1911
10.30699/acadpub.mci
en
jalali
1396
10
1
gregorian
2018
1
1
2
1
online
1
fulltext
en
The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line
Health Services, Quality of Life and Outcomes
Original/Research Article
<strong>Introduction</strong>: Iron oxide nanoparticles, owing to their very small size and superparamagnetic properties, have been considered a potential candidate for several medical applications such as magnetic cell separation, magnetic resonance imaging (MRI), magnetic targeted drug delivery magnetichyperthermia. The present study aimed to synthesize and evaluate the characteristics of superparamagnetic iron oxide nanoparticles (SPIONs) and determine the mechanism by which they induce cell death in the presence of an extremely low-frequency magnetic field (ELMF).<br>
<strong>Methods:</strong> First, SPIONs were synthesized using the chemical co-precipitation method and then characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential. A vibrating-sample magnetometer (VSM) was used to measure the magnetic properties of the nanoparticles. Human MCF-7 breast cancer cells were treated with different concentrations of SPION in the absence and presence of a 50-Hz ELMF for 24 and 48 h. Cytotoxicity and cell viability percentage in the treated cells were measured by the MTT (3-(4,5-di-<br>
methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.<br>
<strong>Results:</strong> DLS and TEM analyses indicated that the SPIONs have an average size of less than 30 nm and they are superparamagnetic. VSM analyses also confirmed the superparamagnetic nature of the nanoparticles. The MTT assay indicated that high concentrations of SPIONs induced death in MCF-7 cells. In the groups treated with ELMF+SPIONs, cell death increased sharply compared with that in the groups treated with each treatment alone (P≤0.05). <br>
<strong>Conclusions: </strong>It seems that a 50-Hz ELMF in the presence of SPIONs led to cell death due to local heating.
Superparamagnetic Iron Oxide, Extremely Low-Frequency Magnetic Fields (ELMFs), X-ray Diffraction
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http://mcijournal.com/browse.php?a_code=A-10-136-1&slc_lang=en&sid=1
Mohammad
Satari
10031947532846001509
10031947532846001509
Yes
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Nazanin
Haghighat
10031947532846001510
10031947532846001510
No
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Fatemeh
Javani Jouni
10031947532846001511
10031947532846001511
No
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Parviz
Abodolmaleki
parviz@modares.ac.ir
10031947532846001512
10031947532846001512
No
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran