en Materials Science and Nanomedicine Review Article <div style="text-align: justify;"><strong>Introduction:</strong> Breast cancer is the leading cause of death in women over the age of 25 worldwide. In spite of the advancements in diagnostic methods, Surgery and invasive sampling of breast tissue are the only definitive diagnostic processes among breast cancer patients. During tumor development, Tumor cells which undergo necrosis or apoptosis release circulation tumor DNA (ctDNA) into the blood. ctDNA molecules are short fragments with 130-200 base pair (bp) carrying the features of genetic and epigenetic tumor tissues. ctDNA is a potential biomarker for detection, accurately monitoring tumor and treatment response. This review was conducted in English, using search engines Pubmed, Medline/Ovid, Scopus, Science Direct, ProQuest, and Web of Science and search terms including &ldquo;Diagnosis or detection of breast cancer&rdquo;, &ldquo;circulating cell free DNA&rdquo; and &ldquo;circulating tumor DNA&rdquo;.<br> <strong>Conclusion:</strong> In this review, we discussed the biological features and clinical applications of circulating tumor DNA in patients with breast cancer.</div> Clinical Application, Clinical Diagnosis, Breast Cancer, Circulating Tumor DNA 0 0 http://mcijournal.com/browse.php?a_code=A-10-33-28&slc_lang=en&sid=1 Mostafa Akbariqomi 10031947532846001023 10031947532846001023 Yes Reza Heidari 10031947532846001024 10031947532846001024 No Hamid Kooshki 10031947532846001025 10031947532846001025 No Sattar Akbari Nakhjavani 10031947532846001026 10031947532846001026 No Paria Sadat Lavasani 10031947532846001027 10031947532846001027 No Moloud Absalan 10031947532846001028 10031947532846001028 No Shilan Shafie 10031947532846001029 10031947532846001029 No Naser Samadi 10031947532846001030 10031947532846001030 No Mahdi Mortazavy 10031947532846001031 10031947532846001031 No Jamshid Motaei 10031947532846001032 10031947532846001032 No