Volume 2, Issue 2 (Multidisciplinary Cancer Investigation 2018)                   Multidiscip Cancer Investig 2018, 2(2): 1-7 | Back to browse issues page


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1- Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran. , f8forouzesh@gmail.com
2- Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
3- Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Biology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
5- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:   (5269 Views)
Introduction: Efforts are made to identify the new potential components such as anticancer drugs. Medicinal plants belonging to Euphorbia genus are widely studied with promising effects. The current study aimed at investigating the effect of ethanolic extract of the root of E. tehranica, grown in Iran, on Caco-2 human colorectal cancer cell-line.
Methods: The Caco-2 cells were treated with different concentrations of the root of E. tehranica ethanolic extract (25 to 1200 μg/mL) at 24 and 48 hours. Cell growth was evaluated with MTT assay. Anticancer activity of the ethanolic extract was assessed by evaluating the cell viability. Cell viability was determined at the wavelength of 570 nm by MTT method. The IC50 (half maximal inhibitory concentration) was determined graphically. One-way ANOVA followed by Tukey’s post-test; and P values <0.05 were considered statistically significant.
Results: The findings showed the significant cytotoxic effect of the root of E. tehranica ethanolic extract against Caco-2 cell-lines (P ≤0.05). Therefore, the viability of Caco-2 cells reduced with the dose and time-dependent manner, when compared with that of the control group. Also, the differences between the IC50 in 24 and 48 hours were 850 and 855 μg/mL, respectively.
Conclusions: It was the first study on the effect of the root of E. tehranica ethanolic extract on human colon cancer cell-lines. The results showed that this extract had the capacity to inhibit proliferation of tumor cells. Therefore, it seems to be a good candidate to defend against cancer cells.
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Select article type: Original/Research Article | Subject: Biomedical Engineering and Technologies
Received: 2018/02/2 | Accepted: 2018/03/23 | ePublished: 2018/04/1

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