Volume 2, Issue 4 (Multidisciplinary Cancer Investigation 2018)
Multidiscip Cancer Investig 2018, 2(4): 16-24 |
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Maria Carolina Ceriani 
1, Pamela Anahi Lendez2 , Lucia Martinez Cuesta3 , Maria Victoria Nieto Farias3 , Hua Min Shen4 , Gertrude Case Buehring4 , Guillermina Laura Dolcini3
1, Pamela Anahi Lendez2 , Lucia Martinez Cuesta3 , Maria Victoria Nieto Farias3 , Hua Min Shen4 , Gertrude Case Buehring4 , Guillermina Laura Dolcini3
1- FCV-UNCPBA-CONICET , cceriani@vet.unicen.edu.ar
2- FCV-UNCPBA
3- Laboratorio de Virología, Facultad de Ciencias Veterinarias, UNCPBA-CONICET
4- The School of Public Health, University of California, Berkeley, California, USA
2- FCV-UNCPBA
3- Laboratorio de Virología, Facultad de Ciencias Veterinarias, UNCPBA-CONICET
4- The School of Public Health, University of California, Berkeley, California, USA
Abstract: (6060 Views)
Abstract
Introduction: Bovine leukemia virus (BLV) causes enzootic bovine leukemia, andis closely related to human T-lymphotropic virus type 1. It expresses microRNAs of unknown function and codes Tax, the protein that mediates malignant transformation. BLV is capable of infecting B- and T-lymphocytes, endothelial cells, and mammary epithelial cells of cattle. Several studies demonstrated the presence of BLV DNA in human tissue, and it is significantly associated with breast cancer in case-control studies using in situ PCR, a highly sensitive and specific technique. The current study was the first report of BLV DNA detection by in situ PCR in tissue from Argentinian females with a diagnosis of breast cancer.
Methods: In situ PCR was done to detect BLV DNA in 85 human FFPE breast cancer tissue samples. The association of BLV DNA and expression of Ki67 and Her-2 was assessed.
Results: BLV DNA presence could be determined in 22.6% of the analyzed samples, and its presence was associated with an increase of the expression and prognosis markers Ki67 (P=0.009) and HER-2 (P=0.044) determined by conventional immunohistochemistry. No statistical significance was observed between the presence or absence of hormonal receptors and the presence of BLV DNA.
Conclusions: The obtained results support the idea that BLV might play a role in malignant tissue transformation.
Introduction: Bovine leukemia virus (BLV) causes enzootic bovine leukemia, andis closely related to human T-lymphotropic virus type 1. It expresses microRNAs of unknown function and codes Tax, the protein that mediates malignant transformation. BLV is capable of infecting B- and T-lymphocytes, endothelial cells, and mammary epithelial cells of cattle. Several studies demonstrated the presence of BLV DNA in human tissue, and it is significantly associated with breast cancer in case-control studies using in situ PCR, a highly sensitive and specific technique. The current study was the first report of BLV DNA detection by in situ PCR in tissue from Argentinian females with a diagnosis of breast cancer.
Methods: In situ PCR was done to detect BLV DNA in 85 human FFPE breast cancer tissue samples. The association of BLV DNA and expression of Ki67 and Her-2 was assessed.
Results: BLV DNA presence could be determined in 22.6% of the analyzed samples, and its presence was associated with an increase of the expression and prognosis markers Ki67 (P=0.009) and HER-2 (P=0.044) determined by conventional immunohistochemistry. No statistical significance was observed between the presence or absence of hormonal receptors and the presence of BLV DNA.
Conclusions: The obtained results support the idea that BLV might play a role in malignant tissue transformation.
Keywords: Leukemia Virus, Bovine, Human Breast Cancer, Direct In Situ PCR, Cell Proliferation, Prognosis
Select article type: Original/Research Article |
Received: 2018/09/1 | Accepted: 2018/09/22 | ePublished: 2018/10/1
Received: 2018/09/1 | Accepted: 2018/09/22 | ePublished: 2018/10/1
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