Multidisciplinary Cancer Investigation
نشریه بین المللی چند تخصصی سرطان
Multidiscip Cancer Investig
Medical Sciences
http://mcijournal.com
1
admin
2476-4922
2538-1911
10.30699/acadpub.mci
en
jalali
1398
7
1
gregorian
2019
10
1
3
4
online
1
fulltext
en
In vitroAnticancer Evaluation of Saponins Obtained From Spirulina platensis on MDA, HepG2, and MCF7 Cell Lines
Original/Research Article
<div style="text-align: justify;"><strong>Introduction: </strong>Microalgae are known for their bioactive compounds with potential applications as antimicrobial, antiaging, and anticancer activities. <em>Spirulina platensi</em>s (<em>S.</em> <em>platensis</em>) is a filamentous and photosynthetic microorganism that has 25 kinds of vitamins and minerals that contain many compounds with biotic activity such as alkaloids, phenolic compounds, terpenoids, and saponins. Saponins are mainly present in plants; while there are few studies about their role in microalgae. This study aims to investigate the anticancer potential of extracted saponins from <em>S. platensis</em>.<br>
<strong>Methods: </strong>Saponins were extracted; using distilled water and n-butanol. The total extracted saponin was dried and weighed. The cellular viability of HepG2, MCF-7, and MDA- MB-123 cell lines was evaluated; using MTT assay after 24 h treatment with 0.02-2 mg/ ml of saponins extracted from <em>S. platensis</em>. Morphology of cell lines was evaluated by invert microscopy.<br>
<strong>Results: </strong>Total saponin extracted from <em>S. platensis </em>was estimated at 28±0.0005 mg/g dry wt. Thin-layer chromatography profiles showed four bands for saponins with Rf values of 0.44, 0.48, 0.50, and 0.55. The cytotoxic activity after 24 h treatment with 0.02-2 mg/ml of saponins was a concentration-dependent manner. The highest toxicity of saponins with IC50=0.22 mg/ml was observed in MDA-MB-123 cells. In HepG2 and MCF-7 cells IC50 value was obtained in 0.35 mg/ml and 0.4 mg/ml, respectively.<br>
<strong>Conclusions</strong>: This is the first report to evaluate the anticancer effects of saponins from <em>S. platensis </em>in liver and breast cancers. The result showed that saponins from <em>Spirulina</em> decrease cancer cellular viability. Therefore, these compounds can be a candidate for anticancer agents.</div>
Microalgae, Antineoplastic Agents, Saponins, Spirulina, Chromatography, Thin Layer
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http://mcijournal.com/browse.php?a_code=A-10-312-1&slc_lang=en&sid=1
Mahboobeh
Akbarizare
Mahboobeh_akbarizare@yahoo.com
10031947532846002316
10031947532846002316
No
Iranian Research Organization for Science and Technology
Hamideh
Ofoghi
ofoghi@irost.ir
10031947532846002317
10031947532846002317
Yes
Iranian Research Organization for Science and Technology
Mahnaz
Hadizadeh
hadizadeh@irost.ir
10031947532846002318
10031947532846002318
No
Iranian Research Organization for Science and Technology