Multidisciplinary Cancer Investigation
نشریه بین المللی چند تخصصی سرطان
Multidiscip Cancer Investig
Medical Sciences
http://mcijournal.com
1
admin
2476-4922
2538-1911
10.30699/acadpub.mci
en
jalali
1399
7
1
gregorian
2020
10
1
4
4
online
1
fulltext
en
Supernatants From Human Osteosarcoma Cultured Cell Lines Induce Modifications in Growth and Differentiation of THP-1 Cells and Phosphoinositide- Specific Phospholipase C Enzymes
Biomedical Engineering and Technologies
Original/Research Article
<div style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong>Int</strong><strong>r</strong><strong>oduction</strong><strong>: </strong>Introduction: Molecular components within the microenvironment act upon cell growth, survival/apoptosis, and proliferation. Immune system cells respond to molecules produced by the tumor and released in the surrounding microenvironment, such as cytokines, chemokines, and growth factors. This study aimed to identify the effects of tumor environment on monocyte-macrophage cell lineage.<br>
<strong>Methods</strong><strong>: </strong>We evaluated morphological and functional changes in THP-1 cells cultured in culture medium mixed with the culture supernatant of one of three different osteosarcoma (OS) cell lines, namely 143B, HS888, and MG-63. We analyzed the effect of supernatants from OS cell lines on morphology and growth of THP-1 cells, and mRNA expression of phosphoinositide-specific phospholipase C (PLC) enzymes. <strong>Results</strong><strong>: </strong>in supernatants from each OS cell line we identified the presence of selected interleukin (TL), TNFa, and GM-CSF. Each OS-derived supernatant differently modified the growth rate of THP-l cells, depending on the cell line. OS supernatants greatly modified the expression panel of PLC enzymes expressed by THP-l cells in the in vitro microenvironment. THP-1 cells differently express PLC enzymes, depending on the origin of the supernatant. The differences in PLCs’ expression induced by OS supernatants resulted in a statistically significant difference in expression of PLCBl and PLCG2 genes.<br>
<strong>Conclusions: </strong>OS supernatants induce the differentiation of THP-1 cells into macrophages. THP-1 cells cultured in OS supernatants expressed different expression panels of PLC enzymes at the mRNA level. The expression panel of PLC enzymes differs during the differentiation of monocyte/macrophage lineage THP-1 cells.</span></span></div>
Phosphoinositides, Signal Transduction, Tumour Microenvironment, Monocyte-Macrophage
1
12
http://mcijournal.com/browse.php?a_code=A-10-430-1&slc_lang=en&sid=1
Martina
Leopizzi
carico@hotmail.com
10031947532846002700
10031947532846002700
No
Department of Medico-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome – Polo Pontino, Latina, Italy
Valeria
Di Maio
valiomon@hotmail.it
10031947532846002701
10031947532846002701
No
Department of Medico-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome – Polo Pontino, Latina, Italy
Carlo
Della Rocca
cdroma@hotmail.com
10031947532846002702
10031947532846002702
No
Department of Medico-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome – Polo Pontino, Latina, Italy
Vincenza Rita
Lo Vasco
ritalovasco@hotmail.it
10031947532846002703
10031947532846002703
Yes
Department of Biomedical, Metabolic and Neural Sciences, Section of Mor- phology, University of Modena and Reggio Emilia, Modena, Italy