Multidisciplinary Cancer Investigation

en Cumulative Effect of a Gold-Poly(o-aminophenol) Nanocomposite and Doxorubicin in Photothermal Therapy, Sonodynamic Therapy, and Chemotherapy of Breast Cancer (MCF-7 Cell Line) Genetics Original/Research Article <div style="text-align: justify;">Introduction: Nowadays, the applications of non-invasive treating cancer methods such as photothermal therapy (PTT) and sonodynamic therapy (SDT) are increasing. These treatments use photo/sonosensitizers, which are activated after being exposed by laser light irradiation and ultrasound (US) exposure, respectively. Methods: Herein, a gold-poly (ortho-aminophenol) nanocomposite (Au-PoAP NC) with a spherical shape, a diameter of 46±8 nm was synthesized and evaluated as an 808-nm laser photosensitizer (with 30% photothermal efficiency) and a sonosensitizer upon US exposure. Additionally, Au-PoAP NC was appraised with doxorubicin (as a chemotherapy agent) for treating breast cancer cells. The MTT test was done for cell-toxicity evaluation of Au-PoAP NC, doxorubicin, and Au-PoAP NC with doxorubicin with or without irradiation and US exposure (separately and synchronously). Results: The results proved that light irradiation and US exposure (separately and synchronously) of Au-PoAP NC with doxorubicin significantly enhanced the cell toxicity in other treatment groups. Moreover, cytotoxicity of Au-PoAP NC, doxorubicin, and Au-PoAP NC with doxorubicin toward MCF-7 cells upon PTT and/or SDT was investigated from the aspect of reactive oxygen species (ROS) formation. Calculating the combination indices revealed that synchronous administration of Au-PoAP NC and doxorubicin and light irradiation and US exposure represented a synergistic therapeutic manner for treating cancer cells. Conclusion: This study proved that the synchronous combination of PTT and SDT using Au-PoAP NC with doxorubicin would be an exemplary approach for treating the breast cancer cell line of MCF-7.</div> Diode laser, Ultrasound waves, Drug delivery, Nanogold, 2-aminophenol 9 20 http://mcijournal.com/browse.php?a_code=A-10-661-1&slc_lang=en&sid=1 paria Tamaddon paria.tmdn@gmail.com 10031947532846003964 10031947532846003964 Yes Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Ghazale Perota 10031947532846003965 10031947532846003965 No Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Rezvan Dehdari Vais dehdari@hotmail.com 10031947532846003966 10031947532846003966 No Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Naghmeh Sattarahmady sattarahmady@yahoo.com 10031947532846003967 10031947532846003967 No Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran