Volume 3, Issue 2 (Multidisciplinary Cancer Investigation 2019)                   Multidiscip Cancer Investig 2019, 3(2): 14-19 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shaikhpour M, Sadeghi A, Yazdian F, Mansoori A, Movafagh A. Anticancer and Apoptotic Effects of Ectoine and Hydroxyectoine on Non-Small Cell Lung Cancer cells: An in-vitro Investigation. Multidiscip Cancer Investig. 2019; 3 (2) :14-19
URL: http://mcijournal.com/article-1-223-en.html
Abstract:   (4629 Views)
Introduction: Streptomyces manage the osmotic stress by collecting compatible solutes or osmolytes. Ectoine, 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, and 5-hydroxyectoine are among the most common osmolytes of this bacteria and have an active role in protecting the genetic material of this microorganism as well as providing resistance to environmental pressures. In this research, the antiproliferative and apoptotic effects of ectoine and 5- hydroxyectoine on human non-small cell lung cancer (NSCLC) cell line (QU-DB) are investigated.
Methods: QU-DB cells were treated by five doses of ectoine and hydroxyectoine for 12, 24 and 48 hours, and the cytotoxic properties were evaluated by MTT assay. Then, the DAPI fluorescent staining was carried out for monitoring of the cells’nuclear morphology. Recognition and quantification of the apoptosis were done; using the Annexin-V-Fluos technique.
Results: It has been verified that both ectoine and hydroxyectoine persuaded apoptosis on QU-DB cells in a dose-dependently manner.
Conclusions: Ectoine and hydroxyectoine are two natural metabolites which induce apoptosis in lung cancer cells. They don’t have any toxic effect on normal cells and can be used as auxiliary drugs or even as a medicine for the treatment of lung cancer after further optimization of the formulations.
Full-Text [PDF 483 kb]   (1138 Downloads)    
Type of Study: Original/Research Article | Subject: basic and translational research
Received: 2019/02/4 | Accepted: 2019/03/11 | ePublished: 2019/04/1

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Multidisciplinary Cancer Investigation

Designed & Developed by : Yektaweb