Volume 2, Issue 4 (Multidisciplinary Cancer Investigation 2018)                   Multidiscip Cancer Investig 2018, 2(4): 1-11 | Back to browse issues page

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Khorshidi S, Palangi A. Strategies, Quality Control and Clinical Applications of Next Generation Sequencing . Multidiscip Cancer Investig. 2018; 2 (4) :1-11
URL: http://mcijournal.com/article-1-205-en.html
Abstract:   (50 Views)
DNA sequencing is one of the great Valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) has revolutionized genomic research and molecular biology, So that the whole human genome can be sequenced with a low cost in several days. NGS technology is similar to the traditional method, Sanger, which detects small DNA fragments by using emitted signals at the time of synthesis of each fragment (from the DNA template), with the difference that NGS has ability to determine the massive simultaneous sequencing in a few days with high accuracy and the results detect directly without the need for electrophoresis. In fact, NGS technology combines a variety of steps such as sample preparation, fragmentation of the sample of the studied genome, attachment of adapter to the ends of the fragments, imaging and data analyses. In recent years, NGS technology has continuously expanded the range of applications in different fields by reducing costs, increasing rates and improving the quality of the data. In this review, we provide the potential applications of the NGS technology by emphasizing the diagnosis of the genetic diseases, identification of several types of cancers, prenatal screening, epigenetic modifications, personalized medicine and pathogens identification.
Full-Text [DOCX 104 kb]   (7 Downloads)    
Type of Study: Review Article | Subject: genetics
Received: 2018/07/16 | Accepted: 2018/11/26 | ePublished: 2018/11/26

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