Volume 1 - Supplement Issue 1: Abstracts of International Tehran Breast Cancer Congress                   Multidiscip Cancer Investig 2017, 1 - Supplement Issue 1: Abstracts of International Tehran Breast Cancer Congress: 0-0 | Back to browse issues page


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Zarei Y, Kazemi Noureini S. Cytotoxicity of Benzyl Isoquinolinic Alkaloids, Palmatine and Corydaline on MCF-7 and MDAMB231 Breast Cancer Cell Lines. Multidiscip Cancer Investig. 2017; 1
URL: http://mcijournal.com/article-1-86-en.html
Abstract:   (1262 Views)
Introduction: Breast cancer is the second and the most common leading cause of death in the world that has attracted special attention in drug design and discovery research projects. Here we investigated the cytotoxic effects of two closely related plant secondary metabolites from the family of benzyl isoquinolinic alkaloids on MCF-7 and MDA-MB231 cell lines.
Materials and Methods: Cytotoxicity of the derivatives in both MCF-7 and MDAMB231 cell lines were measured by using MTT test. Cells were seeded on 96 well plates and were treated with different concentrations of the alkaloid. After 48 hours, MTT was added at final concentration of 0.5 mg/ml and incubated for 4 hours. The purple dye developedby living cells was dissolved in DMSO and measured by Powerwave XS2 and Biotek plate reader. IC50 values were calculated by Gen5 software of the machine using at least 3 independent repeats of the experiment.
Results: MTT tests showed that with increasing concentration of these alkaloids, the percentage of cell survival significantly decreased. The obtained data indicated that Palmatine had an IC50= 186μM in the MCF-7 cell line and 91μM in MDA-MB231 cell line. On the other hand, Corydaline with IC50= 55μM in MCF-7 and 42μM in MDA-MB231 has a stronger cytotoxicity than Palmatine.
Conclusions: Comparing the MTT results, we find that corydaline was stronger than palmatine and it was slightly more cytotoxic in MDA-MB231 than MCF-7.
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Type of Study: Original/Research Article | Subject: genetics
Received: 2017/10/25 | Accepted: 2017/10/25 | ePublished: 2017/10/25

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